TCM Medicinal Plants Showed Antioxidant Activity, Reduced NO and TNF Production, and Increased IL-10 in LPS-Induced RAW-264.7 Cells
DOI:
https://doi.org/10.5327/fst.589Keywords:
antioxidants, anti-inflammatory, phenolic compounds, traditional medicinesAbstract
The aim of this study was to determine the in vitro antioxidant (AA) and anti-inflammatory activity of commercial plant extracts used in traditional Chinese medicine, namely: Curcubita moschata (CMO and CMV), Juniperus chinensis (JC), Peucedanum ostruthium (PO), Pinellia ternata Breit. (PTO and PTV), Rubus coreanus (RC), Rubus chingii Hu (RCH), Solanum tuberosum (STV and ST), and Viola mandshurica (VM). AA was determined by the Folin–Ciocalteu reagent reducing substances (FCRRS), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) methods. In vitro production of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages was determined by Griess reagent (nitric oxide [NO]) and tumor necrosis factor-α/interleukin-10 (TNF-α/IL-10) by Enzyme-Linked Immunosorbent Assay. The PTO, RCH, RC, PTV, and VM extracts had the highest FCRRS and FRAP values. RCH, RC, and VM had the highest ORAC values. All the extracts exhibited anti-inflammatory activity by reducing NO and TNF-α production or increasing IL-10. RC and JC treatments showed the highest inhibition of NO production, 68.63% and 64.91%, respectively. PTO and PTV treatments increased IL-10 production up to four-fold. The Pinellia ternata Breit. extract (PTO) was the most promising among all bioactivity assays performed, as it exhibited electron transfer-based AA activity and demonstrated anti-inflammatory effects by stimulating IL-10 production.
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