Molecular detection of Listeria monocytogenes in refrigerated raw milk and geospatial distribution in Brazilian small farms

Autores

  • Jackieline Sampaio STEINLE Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0000-0002-7366-2449
  • Amanda Bezerra BERTOLINI Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0000-0002-1575-6770
  • Juliano Gonçalves PEREIRA Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0000-0002-8713-7506
  • Antonio Carlos PAES Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0000-0001-7774-927X
  • Laís Pereira da SILVA Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0009-0003-8804-3813
  • Bruna dos Santos LEITE Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0009-0007-3138-8887
  • Talytta Motta RIBEIRO Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0009-0000-5768-603X
  • Isabella Neves AIRES Universidade Estadual Paulista “Júlio de Mesquita Filho”, Medical College, Department of Tropical Diseases and Image Diagnosis, Botucatu, SP, Brazil. https://orcid.org/0000-0001-5285-3150
  • Marcos Vinícius RANGEL Universidade Estadual Paulista “Júlio de Mesquita Filho”, Medical College, Department of Tropical Diseases and Image Diagnosis, Botucatu, SP, Brazil. https://orcid.org/0000-0002-8191-2268
  • Vera Cláudia Magalhães CURCI Biological Institute, Araçatuba, SP, Brazil. https://orcid.org/0000-0003-3393-0493
  • Rafael FAGNANI Universidade Estadual de Londrina, Department of Preventive Veterinary Medicine, Londrina, PR, Brazil. https://orcid.org/0000-0002-7392-2087
  • Thainá Valente BERTOZZO Centro Universitário Sagrado Coração, Bauru, SP, Brazil. https://orcid.org/0000-0002-7971-2021
  • Virgínia Bodelão RICHINI-PEREIRA Adolfo Lutz Institute, Regional Laboratories Center II, Bauru, SP, Brazil. https://orcid.org/0000-0001-8312-7397
  • Simone Baldini LUCHEIS Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Sciences, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, SP, Brazil. https://orcid.org/0000-0002-4842-5281

DOI:

https://doi.org/10.5327/fst.00427

Palavras-chave:

listeriosis, bulk tank milk, bovine, molecular, polymerase chain reaction, DNA

Resumo

Listeriosis is a zoonosis whose etiological agent is the bacterium Listeria monocytogenes. Milk and dairy products are considered excellent substrates for the development of L. monocytogenes. Using conventional PCR, L. monocytogenes was investigated in 102 samples of raw milk from bulk tanks of small farms. Also, microbiological analysis was performed in positive samples by PCR. The primers such as PRS, LM1/LM2, inlA, inlC, and inlJ were used for spatial analysis. PRS indicated a positivity of 20.58%, and inlJ and inlC primers indicated 21.57%. The primers inlA and LM1/LM2 were negative. Our findings indicate it was possible to achieve effective molecular detection of L monocytogenes in the tank milk samples evaluated, in detriment to microbiological culture, in which there was no isolation.

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Publicado

2025-04-07

Como Citar

STEINLE, J. S., BERTOLINI, A. B., PEREIRA, J. G., PAES, A. C., SILVA, L. P. da, LEITE, B. dos S., RIBEIRO, T. M., AIRES, I. N., RANGEL, M. V., CURCI, V. C. M., FAGNANI, R., BERTOZZO, T. V., RICHINI-PEREIRA, V. B., & LUCHEIS, S. B. (2025). Molecular detection of Listeria monocytogenes in refrigerated raw milk and geospatial distribution in Brazilian small farms. Food Science and Technology, 45. https://doi.org/10.5327/fst.00427

Edição

v. 45 (2025)

Seção

Artigos Originais